About Me
About Me
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I am a microbial-molecular biologist, biotechnologist, and natural product investigator with a research background in terpene cyclase enzyme and terpene biosynthesis from Actinobacteria. During my time in the lab, I’ve enjoyed applying my diverse technical skillset to research problems and implementing new methods in investigating the production of secondary metabolites or natural products from a recent genome-sequenced Actinobacteria Stackebrandtia nassauensis DSM 44728. I performed bioinformatics investigation for the terpene synthase gene, based on homology search in S. nassauensis followed by polymerase chain reaction (PCR) based molecular cloning, protein overexpression, immobilized metal ion chromatographic (IMAC) purification using fast protein liquid chromatography (FPLC) and gas chromatography-mass spectrometry (GC-MS) based identification of the terpene metabolites.
I am skilled in microbial bioinformatics, molecular cloning, protein overexpression, protein purification, and characterization, along with several biochemical techniques. I have completed the National Institute of Health's computed-based certified training course on laboratory safety and strictly follow the guidelines of laboratory safety protocols.
Besides the above experiences, I worked on plant histone deacetylase (HDAC) sirtuin 2 (SRT2) and its role in plant stress physiology during my post-graduate dissertation training at the National Institute of Plant Genome Research (NIPGR), New Delhi. During this study, I cloned the HDAC gene in an RNAi vector (pHannibal) and transformed Arabidopsis thaliana by Agrobacterium-mediated floral-dip method and studied in detail the role of sirtuin.
I am currently working as a Post-Doctoral Research Associate at the Department of Pharmacology, School of Medicine, University of Virginia, Charlottesville, Virginia, USA. Currently, I am more focused on gathering knowledge that can be applied to the treatment of chronic inflammation and its detrimental effects on many diseases by finding, characterizing, and modulating the ion channels involved in inflammation, immunity, and tissue homeostasis.
Interests
Interests
Secondary Metabolites
Applied & Industrial Microbiology & Biotechnology
Terpene metabolites
Bioinformatics
Protein Biochemistry
Chromatography
Molecular Biology
Medical Pharmacology
Immunology
Employment
Employment
Postdoctoral Research Associate (November 2024 - to date)
Department of Anesthesiology, School of Medicine, University of Virginia, Charlottesville, Virginia (VA) - 22903, USA.
Postdoctoral Research Associate (March 2023 - October 2024)
Department of Pharmacology, School of Medicine, University of Virginia, Charlottesville, Virginia (VA) - 22903, USA.
Research Assistant (January 2022 - September 2022)
Viral Testing Facility, Forensic Science Laboratory, Mohali, Punjab, India.
Education
Education
Ph.D. (Biochemistry) - 2023
Department of Biochemistry, Panjab University, Chandigarh, India.
M.Sc. (Industrial Microbiology) - 2012
School of Life Sciences, Devi Ahilya Vishwavidyalaya, Indore, Madhya Pradesh, India.
B.Sc. (Biotechnology Honors) - 2010
Department of Biotechnology, St. Xavier's College, Ranchi, Jharkhand, India.
Skills
Skills
Chromatography, spectroscopy, and centrifugation
Chromatography, spectroscopy, and centrifugation
PCR, molecular cloning, and protein overexpression in E. coli and Agrobacterium. RNAi technology (Arabidopsis thaliana)
PCR, molecular cloning, and protein overexpression in E. coli and Agrobacterium. RNAi technology (Arabidopsis thaliana)
Protein purification using Immobilized Metal Affinity Chromatography (IMAC) and Fast Protein Liquid Chromatography (FPLC)
Protein purification using Immobilized Metal Affinity Chromatography (IMAC) and Fast Protein Liquid Chromatography (FPLC)
High Performance Liquid Chromatography (HPLC) and Gas Chromatography - Mass Spectrometry (GC-MS)
High Performance Liquid Chromatography (HPLC) and Gas Chromatography - Mass Spectrometry (GC-MS)
Estimation of biomolecules and enzyme assay
Estimation of biomolecules and enzyme assay
Enrichment and isolation, as well as screening, characterisation, and quantification of microorganisms
Enrichment and isolation, as well as screening, characterisation, and quantification of microorganisms
Electrophoresis, western blotting, northern and southern blotting
Electrophoresis, western blotting, northern and southern blotting
Different immunological tests: ELISA, RIA, Immuno-electrophoresis, Immuno-diffusion, and Immuno-precipitation,
Different immunological tests: ELISA, RIA, Immuno-electrophoresis, Immuno-diffusion, and Immuno-precipitation,
RT-PCR
RT-PCR
Flow-cytometry
Flow-cytometry
Voltage-gated patch-clamp
Voltage-gated patch-clamp
Projects
Projects
Developmental role of sirtuin1 (SRT1) gene in Arabidopsis thaliana
Developmental role of sirtuin1 (SRT1) gene in Arabidopsis thaliana
January 2012 - June 2012; National Insitute of Plant Genome Research, New Delhi, India
Isolation, screening, production and purification of enzyme nattokinase
Isolation, screening, production and purification of enzyme nattokinase
June 2009 - August 2009; Best Biotek Research Lab. Pvt. Ltd, Bengaluru, Karnataka, India
Isolation and characterization of microorganisms from sewage water sample
Isolation and characterization of microorganisms from sewage water sample
May 2008 - June 2008; St. Xavier's College, Ranchi, Jharkhand India
Effect of UV irradiation on protein profile of bacterial isolates of soil
Effect of UV irradiation on protein profile of bacterial isolates of soil
June 2007 - July 2007; St. Xavier's College, Ranchi, Jharkhand India
Fellowships
Fellowships
- University Grants Commission-Basic Science Research-Research Fellowships in Science for Meritorious Students (UGC-BSR-RFSMS) from 2013 to 2018.
- Graduate Aptitude Test for Engineering (GATE) in Life Science in year 2012
Positions
Positions
- Post-Doctoral Research Associate, Department of Anesthesiology, University of Virginia School of Medicine, Charlottesville, Virginia 22903, USA from 01 November 2024 - to date.
- Post-Doctoral Research Associate, Department of Pharmacology, School of Medicine, University of Virginia (UVA), Charlottesville, Virginia (VA)-22908, USA from 08 March 2023 - 31 October 2024.
- Research assistant at Viral Testing Facility, Forensic Science Laboratory, Mohali, Punjab, India from 14 January 2022 - 30 September 2022.
- Managing editor at Syncytia since October 2022 - to date.
- Managing editor at The Applied Biology & Chemistry Journal (TABCJ; eISSN: 2582-8789) since July 2020 - to date.
- Reviewer in PLOS One, Letters in Applied NanoBiosciences, Food Safety and Health, Agriculture, Metabolites, Processes, Agronomy, Medicinal Research Reviews, and Advances in Traditional Medicine.
- Founder and Academic Director (Hon) of the Integrative Centre for Research & Innovation in Biology (ICRIB), Chandigarh, India, since January 2023.
- Founder of Braj Mohan Jha Science Research & Innovation Foundation (BMJSRIF), Chandigarh, India, since October 2022.
- International member of the Society for Applied Microbiology (SAM), London, since 2019 to 2022.
Workshops/ Trainings
Workshops/ Trainings
This section will be updated very soon.
This section will be updated very soon.
Seminars/ Conferences/ Symposiums
Seminars/ Conferences/ Symposiums
This section will be updated very soon.
This section will be updated very soon.
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